Examples of metabolites isolated from plants or generated through biosynthesis and/or
bioconversion by plant cell cultures
Supported by funding in two MIT collaboration projects, methods were developed for purification of pyrrolizidine alkaloids from Jacobaea sp. for ecological and toxicitiy studies. Available on order are: senecionine, seneciphylline, jacobine, erucifoline, acetylseneciphylline, acetylerucifoline, jaconine and jacoline, including their respective N-oxides, at high purity levels.
Supported by funding in an IOP genomics project, Catharanthus roseus cell-lines were optimized for strictosidine production. With support of an IPC project, methods were subsequently developed for isolation of strictosidine and conversion to strictosamide, a potential anti-malarial drug, currently extracted from Nauclea latifolia trees.
Gluco-vanilline and Gluco-vanillylalcohol:
As follow-up of the RAAK Hollandse Vanille project, ExPlant developed methods to produce gluco-vanilline and gluco-vanillylalcohol from vanillin by biotransformation by Catharanthus cultured cells. The new glycosylated products have been isolated by prep-HPLC at a purity > 97%.